Detection range: 96T1.0U/mL - 24 U/mL Purpose: This kit is used to determine the content of proopiomelanocortin (POMC) in serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of squid apigenin (POMC) in the specimen. The microporous plate was coated with purified squid progaurin (POMC) antibody to prepare a solid phase antibody, and the endothelin (POMC) was sequentially added to the microcapsule of the coated monoclonal antibody, and then labeled with HRP. The melanocortin (POMC) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the proopiomelanocortin (POMC) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the concentration of squid proopiomelanocortin (POMC) in the sample was calculated from a standard curve.
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