The Li Jinsong research group of the Institute of Biochemistry and Cellular Sciences, Chinese Academy of Sciences and the Liu Lin research group of Nankai University found that the use of the important factors Zscan4 and Yamanaka in the process of nuclear transfer can not only significantly increase the production of iPS cells (induced pluripotent stem cells) Efficiency, and significantly improved the quality of iPS cells. Related results were published in "Cell Research" a few days ago.
According to reports, the use of somatic cell reprogramming technology to obtain pluripotent stem cells from patients and for autologous transplantation can avoid immune rejection problems. This makes somatic cell reprogramming technology a hot spot in stem cell and regenerative medicine research.
Both nuclear transfer and iPS technology can reprogram somatic cells into pluripotent stem cells, but the two have different reprogramming capabilities. Embryonic stem cells (ESCs) obtained after nuclear transfer have pluripotency similar to normal ESCs, and mice can be obtained by tetraploid blastocyst injection, but most iPS cells do not have this ability.
Recent studies have found that there are many genetic variations in human iPS cells. It can be speculated that the factors involved in nuclear transplantation-induced somatic cell reprogramming may play an important role in the stability of genetic Material. If these factors are applied to iPS technology, the stability of cellular genetic material during reprogramming may be maintained, thereby improving the quality of iPS cells.
The researchers established a screening system and found that Zscan4 can significantly improve the formation efficiency of iPS cells. Further research found that Zscan4 can promote the stabilization of genetic material in the telomere and non-telomere regions of cells during reprogramming, and rapidly extend the telomeres of reprogrammed cells.
Since the intervention of Zscan4 stabilized the genome of reprogrammed cells, the quality of iPS cells was significantly improved. Eleven of the 19 strains (58%) were able to obtain mice completely derived from iPS cells by tetraploid blastocyst injection; and Of the iPS cells obtained by the traditional Yamanaka method, only 1 out of 11 strains were able to obtain mice with complete iPS cells.
Relevant experts believe that this research provides new ideas for obtaining iPS cell research through the Yamanaka method, and provides an important theoretical basis for obtaining safer iPS cells and applying them to regenerative medicine.
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