English name: NBT; Nitroblue tetrazolium chloride; Nitroblue tetrazolium; 2,2-Bis (4-Nitrophenyl) -5,5-diphenyl-3,3- (3,3-dimethoxy-4,4-diphenylene) ditetrazolium chloride
Other names: Nitro blue tetrazolium; Nitro blue tetrazolium; Nitro BT; Nitro tetrazolium chloride; Nitro tetrazolium chloride
CAS number: 298-83-9
C40H30Cl2N10O6 = 817.65
Level: BR
Content: ≥98.0%
Methanol dissolution test: qualified absorbance Em (Lambda Max, methanol):> 54000
Properties: light yellow powder or yellow needle crystal. There is no obvious instability to light. Easy to absorb moisture. Soluble in water and ethanol, slightly soluble in ethyl acetate, insoluble in ether. The reduced product (Diformazan) is a black-purple crystalline powder, soluble in most organic solvents. poisonous. Carcinogenic potential and irritating use: substrates for dehydrogenases and other oxidases. For the determination of glucose-6-phosphate dehydrogenase. It is also a precipitation reagent for clotting factor I (fibrinogen). It is also one of the substrates of alkaline phosphatase, which produces an insoluble blue product under the catalysis of alkaline phosphatase. Commonly used in immunohistochemistry (IHC), immunocytochemistry (ICC) and Western blot (WB) experiments. Quantification of oxidoreductase and steroids. Verification of succinate dehydrogenase in histochemistry. Determination of isozymes in clinical chemistry. Used as a cell stain, because it can be reduced to blue-purple azo compounds (Diformazan) by dehydrogenase in malignant cells.
Storage: 2 ～ 8 ℃, protected from light
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