1. Separate and analyze the component content of the drug: Since the drug often contains components, the chromatographic method is a method that can be separated and quantitative. Such as fat-soluble vitamin tablets, containing chromium vitamins, C18 column and methanol mobile phase containing 1% ammonium carbonate. The vitamin B6, D1, A, E can be separated and the content can be determined at one time. Another example is analgesics or antipyretics. This method can also be used to separate and measure the content of each component.
2. Intermediate control in drug production: When producing antibiotics, we must first understand the existence of the main body and by-products in the fermentation broth. For example: the fermentation products of penicillin, tetracycline, cephalosporin, erythromycin, daunorubicin, gentamicin, etc., can be analyzed by the mobile phase prepared by C18 column and methanol and buffer or by ion pair method The content of the product. For procaine amide, theophylline, etc., the main content can be determined, and for nitroglycerin, the main content and its degradation products can be determined to ensure the heat of the drug.
3. Analyze the residual amount of the drug in the body: It is one of the main contents of the recent research on the efficacy of new drugs. For example, oridonin is a component in plants, which has anti-cancer effects, but it is highly toxic. It is very important to determine its content in serum. Now you can use the C18 column and methanol water as the mobile phase to determine the serum content after treatment to obtain the blood content. If the content of sulfonamides in serum and urine is not allowed to be too large, C18 column and acidic ethanol can be used as the mobile phase. After determining the content, the most appropriate dosage can be estimated. Another example is tetracycline, barbituric acid, analgesics, antipyretics, diabetes drugs, etc., the content in blood or urine can be measured using C18 column with methanol and water as mobile phases. Therefore, the application of reversed-phase chromatography is extremely wide.
4. Determine the metabolites of drugs in various organs, especially study the existence of metabolites of drugs in serum. Such as measuring keto acid and glutaric acid in serum. The measurement of metabolites in urine is convenient and does not require complicated pretreatment. Because studying the components in urine can identify the metabolic function of patients, it has important reference value for pathological research or bed diagnosis.
For thousands of years, Chinese medicine has been handed down from generation to generation, and it has also shown miraculous curative effects in the treatment of diseases, occupying an important position in world medicine. However, the complexity and diversity of traditional Chinese medicine ingredients also restrict the development and clinical application of traditional Chinese medicine preparations. The variety of traditional Chinese medicine is not new and the quality of traditional Chinese medicine is far from scientific quantification. Testing equipment. The author reviewed the data and summarized the wide application of HPLC method in the analysis of traditional Chinese medicine preparations. It has the advantages of good separation performance, wide application range, fast analysis speed, good selectivity, high sensitivity, and low sample consumption. With the gradual development and improvement of combined technologies with mass spectrometry and tandem mass spectrometry, high-performance liquid chromatography will definitely play an important role in the identification of traditional Chinese medicine, analysis of active ingredients of traditional Chinese medicine, quality control of production of traditional Chinese medicine and its preparations, etc. The first choice for separation, identification and content determination has a broad application prospect. In recent years, the HPLC method has greatly developed in terms of instruments, detectors, and fixed equals. This has greatly promoted the application of pharmaceutical analysis in Chinese and Western medicines, especially for Chinese medicine, a great treasure trove of medicine. There is a difficulty in a quality index, and HPLC method can solve this problem. However, each analysis technique has its use range and method limitations. Now it is mainly chromatography-chromatography, chromatography-spectrum, high-performance liquid chromatography-mass spectrometry, column switching technology and high-performance liquid chromatography. The combination of high performance liquid chromatography such as Fourier transform infrared absorption spectroscopy and other technologies is the main development direction of high performance liquid chromatography in the future, and it will also be an important way to standardize and internationalize the quality in the future.
Hair Dryer,best hair dryer,hair dryer price,LCD hair dryer
Zhejiang Hisun Electrical Appliance Co.,Ltd , https://www.cn-hisun.com