Abstract: A spectrophotometer uses a light source that can generate multiple wavelengths. A series of spectroscopic devices are used to generate a light source of a specific wavelength. After the light source passes through the tested sample, part of the light source is absorbed, and the absorbance value of the sample is calculated to be converted into The concentration of the sample. The absorbance of the sample is proportional to the concentration of the sample.
Spectrophotometer spectral distribution range:
Including the visible range of the wavelength range of 400 ~ 760nm and the ultraviolet range of the wavelength range of 200 ~ 400nm. Different light sources have their own unique emission spectrum, so different luminous bodies can be used as the light source of the instrument.
Emission spectrum of tungsten lamp: 400-760nm wavelength spectrum emitted by tungsten lamp light source. After the light is refracted by a prism, a continuous chromatogram composed of red, orange, yellow, green, blue, indigo, and violet can be obtained; the chromatogram can be used as The light source of the visible light spectrophotometer.
Emission spectrum of hydrogen lamp (or deuterium lamp): The hydrogen lamp can emit a spectrum of 185 ～ 400nm wavelength and can be used as the light source of ultraviolet photometer.
Substance absorption spectrum
(1) If a solution of a substance is placed between the light source and the prism, the spectrum displayed on the screen at this time is no longer the spectrum of the light source, and it appears several dark lines, that is, some wavelengths in the emission spectrum of the light source The light absorbed by the solution disappears, and the spectrum absorbed by the solution is called the absorption spectrum of the solution. The absorption spectrum of different substances is different. Therefore, according to the absorption spectrum, the substances contained in the solution can be identified. The absorption of the substance spectrum
(2) When the light passes through a solution of a certain substance, the intensity of the transmitted light is weakened. Because part of the light is reflected or scattered on the surface of the solution, part of the light is absorbed by the substance that makes up the solution, and only part of the light can be transmitted Solution. Incident light = reflected light, dispersed light, absorbed light, and transmitted light. If we use distilled water (or the solvent that makes up this solution) as a "blank" to correct the loss of incident light caused by reflection, dispersion, and other factors, then: incident light = absorption Light ten through light
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