Test report of Chenhang company using Cometro (US Connaught) high performance liquid chromatography system to detect sodium benzoate and saccharin sodium
1. Experimental conditions Cometro (U.S. Connaught) high performance liquid chromatography system; Cometro (U.S. Convo) 6000 PVW UV detector, wavelength = 230nm; Cometro (U.S. Cono) 6000 LDI Pump; Scienhome (Chen Hang) YWG C18 250 * 4.6mm chromatographic column; mobile phase: methanol-0.02mol / L ammonium acetate = 10: 90; flow rate: 1.0ml / min; sample: 0.5mg / ml sodium benzoate + 0.5mg / ml sodium saccharin 1: 1 mixed sample; Injection volume: 5ul.
2. Experimental results Retention Time Area Theoretical plates (USP) Resolution (USP) Asymmetry 10.209 2648606 8773 0.00000 1.2052215.931 562371 9736 10.57742 1.18033 3. Discussion of the results It can be seen from the figure that the peak shape is good, the column efficiency is high, the resolution is good, and the retention time is moderate .
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